DNA pol δ cat rabbit pAb

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012],

Product Specifications

Background

Product Name Alternative

POLD1; POLD; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125

UniProt

P28340

Swiss Prot

P28340

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human POLD1. AA range:1051-1100

Target

DNA pol δ cat

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

110 124kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

110 124kD

Fragment

IgG

Subcellular Location

Nucleus . Colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739) . After UV irradiation, recruited to DNA damage sites within 2 hours, independently on the cell cycle phase, nor on PCNA ubiquitination. This recruitment requires POLD3, PCNA and RFC1-replication factor C complex (PubMed:20227374, PubMed:22801543) . .