CD31 (phospho Tyr713) rabbit pAb

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],

Product Specifications

Background

Product Name Alternative

PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

UniProt

P16284

Swiss Prot

P16284

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713. AA range:686-735

Target

CD31

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

150kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

150kD

Fragment

IgG

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation. .; [Isoform Long]: Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. .; [Isoform Delta15]: Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.