What is the catalog number for PDHK1 rabbit pAb?

The catalog number for PDHK1 rabbit pAb is ES6557.

What sizes are available for PDHK1 rabbit pAb?

PDHK1 rabbit pAb is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does PDHK1 rabbit pAb come in?

PDHK1 rabbit pAb is available in 2 different pack sizes.

Is PDHK1 rabbit pAb in stock?

PDHK1 rabbit pAb is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures PDHK1 rabbit pAb?

PDHK1 rabbit pAb is manufactured by ELK.

What is the estimated delivery time for PDHK1 rabbit pAb?

Estimated delivery time for PDHK1 rabbit pAb is approximately 10 business day(s), depending on your location.

PDHK1 rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. function: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. similarity: Contains 1 PH domain. similarity: Contains 1 protein kinase domain. subcellular location: Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. subunit: Interacts with TUSC4. tissue specificity: Appears to be expressed ubiquitously.

Product Specifications

Background

Catalytic activity:ATP + a protein = ADP + a phosphoprotein., function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity) . Isoform 3 is catalytically inactive., PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily., similarity:Contains 1 PH domain., similarity:Contains 1 protein kinase domain., subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane., subunit:Interacts with TUSC4., tissue specificity:Appears to be expressed ubiquitously.

UniProt

O15530

Swiss Prot

O15530

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human PDK1. AA range:1-50

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

60kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

60kD

Fragment

IgG

Subcellular Location

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resul