Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

PDHK1 (phospho Tyr9) rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. function: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. similarity: Contains 1 PH domain. similarity: Contains 1 protein kinase domain. subcellular location: Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. subunit: Interacts with TUSC4. tissue specificity: Appears to be expressed ubiquitously.

Product Specifications

Background

Catalytic activity:ATP + a protein = ADP + a phosphoprotein., function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity) . Isoform 3 is catalytically inactive., PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily., similarity:Contains 1 PH domain., similarity:Contains 1 protein kinase domain., subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane., subunit:Interacts with TUSC4., tissue specificity:Appears to be expressed ubiquitously.

Synonyms

PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

Gene ID

5170

UniProt

O15530

Cellular Locus

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resul

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Tyr9. AA range:1-50

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

IHC, IF, ELISA

Validated Applications

IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES6556-1.pdf

Subcellular Location

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resul

Other Product Names

PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

Gene ID (Human)

5170

SwissProt (Human)

O15530

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

RAD51C sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
38423111 3 x 1.0 µg

RAD51C sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

Ask
View Details
Matrilin-2 shRNA (h) Lentiviral Particles
sc-45447-V 200 µL

Matrilin-2 shRNA (h) Lentiviral Particles

Ask
View Details
NAA60 Antibody
DF13864-01 100 µL

NAA60 Antibody

Ask
View Details
NAA60 Antibody
DF13864-02 200 µL

NAA60 Antibody

Ask
View Details
frizzled-10 Lentiviral Activation Particles (m2)
sc-430086-LAC-2 200 µL

frizzled-10 Lentiviral Activation Particles (m2)

Ask
View Details
Recombinant Daucus carota Peroxidase 6
MBS1174691-01 0.02 mg (E-Coli)

Recombinant Daucus carota Peroxidase 6

Ask
View Details