PARK2 rabbit pAb

The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

O60260

Swiss Prot

O60260

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Parkin. AA range:1-50

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

52kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

52kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytosol . Nucleus . Endoplasmic reticulum . Mitochondrion . Mitochondrion outer membrane . Cell projection, neuron projection . Cell junction, synapse, postsynaptic density . Cell junction, synapse, presynapse . Mainly localizes in the cytosol (PubMed:19029340, PubMed:19229105) . Co-localizes with SYT11 in neutrites (PubMed:12925569) . Co-localizes with SNCAIP in brainstem Lewy bodies (PubMed:10319893, PubMed:11431533) . Translocates to dysfunctional mitochondria that have lost the mitochondrial membrane potential; recruitment to mitochondria is PINK1-dependent (PubMed:24898855, PubMed:18957282, PubMed:19966284, PubMed:23620051) . Mitochondrial localization also gradually increases with cellular growth (PubMed:22082830) . .