NuMA rabbit pAb

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013],

Product Specifications

Background

UniProt

Q14980

Swiss Prot

Q14980

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human NUMA1. AA range:334-383

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

240kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

240kD

Fragment

IgG

Subcellular Location

Nucleus . Nucleus, nucleoplasm . Nucleus matrix . Chromosome . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cell cortex . Cell membrane ; Lipid-anchor ; Cytoplasmic side . Lateral cell membrane . Mitotic cell cycle-dependent shuttling protein that relocalizes from the interphase nucleus to the spindle poles and cell cortex (PubMed:1541636, PubMed:10811826) . The localization to the spindle poles is regulated by AAAS (PubMed:26246606) . In interphase, resides in the nuclear matrix (PubMed:1541630, PubMed:1541636, PubMed:23921553) . In prophase, restricted to the interchromatin or condensed chromosome space (PubMed:10811826) . In prometaphase, after nuclear envelope disassembly, forms aggregates