YB-1 (phospho Ser102) rabbit pAb

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015],

Product Specifications

Background

Product Name Alternative

YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-

UniProt

P67809

Swiss Prot

P67809

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human YB1 around the phosphorylation site of Ser102. AA range:68-117

Target

YB-1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

36kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

36kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Cytoplasmic granule . Secreted . Secreted, extracellular exosome . Predominantly cytoplasmic in proliferating cells (PubMed:12604611) . Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551) . Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427) . Shuttles between nucleus and cytoplasm (PubMed:25229427) . Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541) . Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673) . .