Moesin/Ezrin/Radixin (phospho Thr558) rabbit pAb

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

UniProt

P26038/P35241/P15311

Swiss Prot

P26038/P35241/P15311

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558. AA range:524-573

Target

Moesin/Ezrin/Radixin

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

67kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

67kD

Fragment

IgG

Subcellular Location

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity) . Localizes at the uropods of T lymphoblasts. .