MDM2 (phospho Ser166) rabbit pAb

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],

Product Specifications

Background

UniProt

Q00987

Swiss Prot

Q00987

Reactivity

Human; Mouse; Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser166. AA range:132-181

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

90kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

90kD

Fragment

IgG

Subcellular Location

Nucleus, nucleoplasm. Cytoplasm . Nucleus, nucleolus. Nucleus . Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF (P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.