MCM4 (phospho Ser54) rabbit pAb
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri
Product Specifications
Background
Synonyms
MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21
Gene ID
4173
UniProt
P33991
Cellular Locus
Nucleus. Chromosome. Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses..
Host
Rabbit
Species Reactivity
Human,Mouse,Monkey
Reactivity
Human; Mouse; Monkey
Immunogen
The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69
Clonality
Polyclonal
Isotype
IgG
Source
Rabbit
Applications
WB, IHC, IF, ELISA
Validated Applications
WB,IHC,IF,ELISA
Stability
-20°C/1 year
Concentration
1 mg/mL
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
85kD
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES6207-1.pdf
Observed Molecular Weight
85 kD
Subcellular Location
Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
Other Product Names
MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21
Gene ID (Human)
4173
SwissProt (Human)
P33991
Available Sizes
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