MCM4 (phospho Ser54) rabbit pAb
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri
Product Specifications
Background
UniProt
P33991
Swiss Prot
P33991
Reactivity
Human; Mouse; Monkey
Immunogen
The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC; IF; ELISA
Concentration
1 mg/ml
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
85kD
Storage Conditions
-20°C/1 year
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES6207-1.pdf
Observed Molecular Weight
85kD
Fragment
IgG
Subcellular Location
Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
Other Product Names
MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21
Gene ID (Human)
4173
Available Sizes
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