HSL (phospho Ser855) rabbit pAb

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

LIPE; Hormone-sensitive lipase; HSL

UniProt

Q05469

Swiss Prot

Q05469

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554. AA range:520-569

Target

HSL

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

81kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

81kD

Fragment

IgG

Subcellular Location

Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959) . Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity) . .