IRF-3 (phospho Ser386) rabbit pAb

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

Product Specifications

Background

Product Name Alternative

IRF3; Interferon regulatory factor 3; IRF-3

UniProt

Q14653

Swiss Prot

Q14653

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser386. AA range:352-401

Target

IRF-3

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300

Buffer

-20°C/1 year

Molecular Weight

48-55kd

Storage Conditions

-20°C/1 year

Observed Molecular Weight

48-55kd

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Mitochondrion . Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757) . When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757) . Recruited to mitochondria via TOMM70:HSP90AA1 upon Sendai virus infection (PubMed:25609812) . .