AIRE-1 (phospho Ser156) rabbit pAb

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012],

Product Specifications

Background

Synonyms

AIRE; APECED; Autoimmune regulator; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; APECED protein

Gene ID

326

UniProt

O43918

Cellular Locus

Nucleus. Cytoplasm. Predominantly nuclear but also cytoplasmic (PubMed:11274163, PubMed:14974083). Found in nuclear body-like structures (dots) and in a filamentous vimentin-like pattern (PubMed:11274163, PubMed:14974083, PubMed:26084028). Associated with tubular structures (PubMed:11274163, PubMed:14974083)..

Host

Rabbit

Species Reactivity

Human,Rat,Mouse,

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human AIRE around the phosphorylation site of Ser156. AA range:126-175

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, ELISA

Validated Applications

WB,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

50kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES5771-1.pdf

Observed Molecular Weight

50 kD

Subcellular Location

Nucleus . Cytoplasm . Predominantly nuclear but also cytoplasmic (PubMed:11274163, PubMed:14974083) . Found in nuclear body-like structures (dots) and in a filamentous vimentin-like pattern (PubMed:11274163, PubMed:14974083, PubMed:26084028) . Associated with tubular structures (PubMed:11274163, PubMed:14974083) . .

Other Product Names