UBN-1 rabbit pAb

Function: Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-independent chromatin assembly. similarity: Belongs to the ubinuclein family. subcellular location: Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts. Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with TJP1 and CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis. subunit: Component of a complex that includes at least ASF1A, CABIN1, HIRA, histone H3. 3 and UBN1. Interacts with Epstein-Barr virus BZLF1. Interacts with HIRA (via WD repeat domain); the interaction is direct. Interacts with ASF1A, CEBPA, TJP1, TJP2 and TJP3. tissue specificity: Ubiquitous. Also expressed in numerous tumors and cancer cell lines.

Product Specifications

Background

Function:Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-independent chromatin assembly., similarity:Belongs to the ubinuclein family., subcellular location:Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts. Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with TJP1 and CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis., subunit:Component of a complex that includes at least ASF1A, CABIN1, HIRA, histone H3.3 and UBN1. Interacts with Epstein-Barr virus BZLF1. Interacts with HIRA (via WD repeat domain) ; the interaction is direct. Interacts with ASF1A, CEBPA, TJP1, TJP2 and TJP3., tissue specificity:Ubiquitous. Also expressed in numerous tumors and cancer cell lines.

UniProt

Q9NPG3

Swiss Prot

Q9NPG3

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human Ubinuclein. AA range:161-210

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Nucleus, nucleoplasm. Nucleus, PML body. Cell junction, tight junction. Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts. Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with TJP1 and CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis.