Eps15 (phospho Tyr849) rabbit pAb

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009],

Product Specifications

Background

Product Name Alternative

EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p

UniProt

P42566

Swiss Prot

P42566

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849)

Target

Eps15

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

100kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

100kD

Fragment

IgG

Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes. .; [Isoform 2]: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with HGS on bilayered clathrin coats on endosomes.