4E-BP1 (phospho Thr46) rabbit pAb
Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],
Product Specifications
Background
Synonyms
Gene ID
1978
UniProt
Q13541
Cellular Locus
Nucleoplasm,cytoplasm,cytosol,protein complex,
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Reactivity
Human; Mouse; Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45. AA range:13-62
Clonality
Polyclonal
Isotype
IgG
Source
Rabbit
Applications
WB, IHC, IF, ELISA
Validated Applications
WB,IHC,IF,ELISA
Stability
-20°C/1 year
Concentration
1 mg/mL
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
18kD
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES5077-1.pdf
Observed Molecular Weight
18 kD
Subcellular Location
Nucleoplasm, cytoplasm, cytosol, protein complex
Other Product Names
Gene ID (Human)
1978
SwissProt (Human)
Q13541
Available Sizes
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