DGK-θ rabbit pAb

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P52824

Swiss Prot

P52824

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human DGKQ. AA range:691-740

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight

101kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

101kD

Fragment

IgG

Subcellular Location

Cytoplasm . Cytoplasm, cytosol . Cell membrane . Cell junction, synapse . Cytoplasm, cytoskeleton . Nucleus . Nucleus speckle . Nucleus matrix . Translocates to the plasma membrane in response to steroid hormone receptor stimulation (PubMed:15632189) . Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH (PubMed:15632189) . Translocates to the nucleus in response to thrombin stimulation (Probable) . Association with the nuclear matrix is regulated by nerve growth factor (By similarity) . .