Casein Kinase Iα (phospho Thr321) rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. function: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. similarity: Contains 1 protein kinase domain. subunit: Monomer. Interacts with the Axin complex.

Product Specifications

Background

Catalytic activity:ATP + a protein = ADP + a phosphoprotein., function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily., similarity:Contains 1 protein kinase domain., subunit:Monomer. Interacts with the Axin complex.

UniProt

P48729

Swiss Prot

P48729

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Casein Kinase I alpha around the phosphorylation site of Tyr321. AA range:287-336

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

35kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

35kD

Fragment

IgG

Subcellular Location

Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . Nucleus speckle . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, spindle . Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688) . .