CPLA2-ε rabbit pAb

Catalytic activity: Phosphatidylcholine + H (2)O = 1-acylglycerophosphocholine + a carboxylate. Domain: The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca (2+). enzyme regulation: Stimulated by cytosolic Ca (2+). function: Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. similarity: Contains 1 C2 domain. similarity: Contains 1 PLA2c domain. subcellular location: Translocates to lysosomal membranes in a calcium-dependent fashion.

Product Specifications

Background

Catalytic activity:Phosphatidylcholine + H (2) O = 1-acylglycerophosphocholine + a carboxylate., domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca (2+) ., enzyme regulation:Stimulated by cytosolic Ca (2+) ., function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position., similarity:Contains 1 C2 domain., similarity:Contains 1 PLA2c domain., subcellular location:Translocates to lysosomal membranes in a calcium-dependent fashion.

UniProt

Q3MJ16

Swiss Prot

Q3MJ16

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human PLA2G4E. AA range:401-450

Clonality

Polyclonal

Source

Rabbit

Applications

IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cytoplasm, cytosol . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell membrane ; Peripheral membrane protein; Cytoplasmic side . Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates. .