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CPLA2-ε rabbit pAb

Catalytic activity: Phosphatidylcholine + H (2)O = 1-acylglycerophosphocholine + a carboxylate. Domain: The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca (2+). enzyme regulation: Stimulated by cytosolic Ca (2+). function: Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. similarity: Contains 1 C2 domain. similarity: Contains 1 PLA2c domain. subcellular location: Translocates to lysosomal membranes in a calcium-dependent fashion.

Product Specifications

Background

Catalytic activity:Phosphatidylcholine + H (2) O = 1-acylglycerophosphocholine + a carboxylate., domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca (2+) ., enzyme regulation:Stimulated by cytosolic Ca (2+) ., function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position., similarity:Contains 1 C2 domain., similarity:Contains 1 PLA2c domain., subcellular location:Translocates to lysosomal membranes in a calcium-dependent fashion.

Synonyms

PLA2G4E; Cytosolic phospholipase A2 epsilon; cPLA2-epsilon; Phospholipase A2 group IVE

Gene ID

123745

UniProt

Q3MJ16

Cellular Locus

Cytoplasm, cytosol. Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Cell membrane ; Peripheral membrane protein; Cytoplasmic side. Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates..

Host

Rabbit

Species Reactivity

Human,Rat,Mouse,

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human PLA2G4E. AA range:401-450

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

IHC, IF, ELISA

Validated Applications

IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES4712-1.pdf

Subcellular Location

Cytoplasm, cytosol . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell membrane ; Peripheral membrane protein; Cytoplasmic side . Targeted to clathrin-independent endocytotic vesicles through binding to phosphoinositides, especially phosphatidylinositol 4,5-bisphosphates. .

Other Product Names

PLA2G4E; Cytosolic phospholipase A2 epsilon; cPLA2-epsilon; Phospholipase A2 group IVE

Gene ID (Human)

123745

SwissProt (Human)

Q3MJ16

Available Sizes

Curated Selection

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