NUDC (phospho Ser326) rabbit pAb

This gene encodes a nuclear distribution protein that plays an essential role in mitosis and cytokinesis. The encoded protein is involved in spindle formation during mitosis and in microtubule organization during cytokinesis. Pseudogenes of this gene are found on chromosome 2. [provided by RefSeq, Feb 2012],

Product Specifications

Background

UniProt

Q9Y266

Swiss Prot

Q9Y266

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human NudC around the phosphorylation site of Ser326. AA range:282-331

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

45kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

45kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytoskeleton. Nucleus . Cytoplasm, cytoskeleton, spindle . Midbody . In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. Localizes to the mitotic spindle in a EML4-dependent manner (PubMed:25789526) . .