Cdk9 (phospho Thr186) rabbit pAb

Cyclin dependent kinase 9 (CDK9) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

CDK9; CDC2L4; TAK; Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit

UniProt

P50750

Swiss Prot

P50750

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186. AA range:152-201

Target

Cdk9

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

42kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

42kD

Fragment

IgG

Subcellular Location

Nucleus. Cytoplasm. Nucleus, PML body. Accumulates on chromatin in response to replication stress. Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated.