ACK (phospho Tyr284) rabbit pAb

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008],

Product Specifications

Background

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP) -stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Product Name Alternative

TNK2; ACK1; Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2

UniProt

Q07912

Swiss Prot

Q07912

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. AA range:250-299

Target

ACK

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

120kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

120kD

Fragment

IgG

Subcellular Location

Cell membrane . Nucleus . Endosome . Cell junction, adherens junction . Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasmic vesicle, clathrin-coated vesicle . Membrane, clathrin-coated pit . Cytoplasm, perinuclear region . Cytoplasm, cytosol . The Tyr-284 phosphorylated form is found both in the membrane and nucleus (By similarity) . Co-localizes with EGFR on endosomes (PubMed:20333297) . Nuclear translocation is CDC42-dependent (By similarity) . Detected in long filamentous cytosolic structures where it co-localizes with CTPS1 (By similarity) . .