Glut4 rabbit pAb

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

SLC2A4; GLUT4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive; GLUT-4

UniProt

P14672

Swiss Prot

P14672

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from the N-terminal region of human SLC2A4. AA range:21-70

Target

Glut4

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

56kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

56kD

Fragment

IgG

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Endomembrane system ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized (PubMed:8300557) . The dileucine internalization motif is critical for intracellular sequestration (PubMed:8300557) . Insulin stimulation induces translocation to the cell membrane (By similarity) . .