Apaf-1 rabbit pAb
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding Cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Product Specifications
Background
Product Name Alternative
APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
UniProt
O14727
Swiss Prot
O14727
Reactivity
Human; Mouse; Rat
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550
Target
APAF1
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC; IF; ELISA
Concentration
1 mg/ml
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Buffer
-20°C/1 year
Molecular Weight
135kD
Storage Conditions
-20°C/1 year
Observed Molecular Weight
135kD
Fragment
IgG
Subcellular Location
Cytoplasm .
Other Product Names
APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
Gene ID (Human)
317
Available Sizes
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