Apaf-1 rabbit pAb
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding Cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Product Specifications
Background
Synonyms
APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
Gene ID
317
UniProt
O14727
Cellular Locus
Cytoplasm.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Reactivity
Human; Mouse; Rat
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550
Clonality
Polyclonal
Isotype
IgG
Source
Rabbit
Applications
WB, IHC, IF, ELISA
Validated Applications
WB,IHC,IF,ELISA
Stability
-20°C/1 year
Concentration
1 mg/mL
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
135kD
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES4108-1.pdf
Observed Molecular Weight
135 kD
Subcellular Location
Cytoplasm .
Other Product Names
APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1
Gene ID (Human)
317
SwissProt (Human)
O14727
Available Sizes
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
