CD177 rabbit pAb

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014],

Product Specifications

Background

This gene encodes a glycosyl-phosphatidylinositol (GPI) -linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]

Product Name Alternative

CD177; NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177

UniProt

Q8N6Q3

Swiss Prot

Q8N6Q3

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human CD177. AA range:361-410

Target

CD177

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

46kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

46kD

Fragment

IgG

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor . Membrane raft ; Lipid-anchor, GPI-like-anchor . Secreted . Cytoplasmic granule membrane . Cell projection, lamellipodium . Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308) . In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208) . Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208) . Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980) . .