PEPCK-C rabbit pAb
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008],
Product Specifications
Background
Synonyms
PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Phosphoenolpyruvate carboxylase
Gene ID
5105
UniProt
P35558
Cellular Locus
Cytoplasm, cytosol. Endoplasmic reticulum. Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum..
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Reactivity
Human; Mouse; Rat
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human PCK1. AA range:491-540
Clonality
Polyclonal
Isotype
IgG
Source
Rabbit
Applications
WB, IHC, IF, ELISA
Validated Applications
WB,IHC,IF,ELISA
Stability
-20°C/1 year
Concentration
1 mg/mL
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
65kD
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES3966-1.pdf
Observed Molecular Weight
65 kD
Subcellular Location
Cytoplasm, cytosol . Endoplasmic reticulum . Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum. .
Other Product Names
PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Phosphoenolpyruvate carboxylase
Gene ID (Human)
5105
SwissProt (Human)
P35558
Available Sizes
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