Keap-1 rabbit pAb

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19

UniProt

Q14145

Swiss Prot

Q14145

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460

Target

Keap1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

70kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

70kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839) . In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972) . .