Fascin 1 rabbit pAb

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011],

Product Specifications

Background

Product Name Alternative

FSCN1; FAN1; HSN; SNL; Fascin; 55 kDa actin-bundling protein; Singed-like protein; p55

UniProt

Q16658

Swiss Prot

Q16658

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human FSCN1. AA range:261-310

Target

Fascin

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

55kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

55kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytosol . Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, stress fiber . Cell projection, filopodium . Cell projection, invadopodium . Cell projection, microvillus . Cell junction . Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F-actin at the transitional domain of the axonal growth cone (By similarity) . .