SMIF rabbit pAb

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Product Specifications

Background

UniProt

Q9NPI6

Swiss Prot

Q9NPI6

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human DCP1A. AA range:111-160

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

75kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

75kD

Fragment

IgG

Subcellular Location

Cytoplasm, P-body . Nucleus . Co-localizes with NANOS3 in the processing bodies (By similarity) . Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915) . Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524) . .