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SMIF rabbit pAb

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Product Specifications

Background

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Synonyms

DCP1A; SMIF; mRNA-decapping enzyme 1A; Smad4-interacting transcriptional co-activator; Transcription factor SMIF

Gene ID

55802

UniProt

Q9NPI6

Cellular Locus

Cytoplasm, P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524)..

Host

Rabbit

Species Reactivity

Human,Mouse

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human DCP1A. AA range:111-160

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

75kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES3467-1.pdf

Observed Molecular Weight

75 kD

Subcellular Location

Cytoplasm, P-body . Nucleus . Co-localizes with NANOS3 in the processing bodies (By similarity) . Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915) . Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524) . .

Other Product Names

DCP1A; SMIF; mRNA-decapping enzyme 1A; Smad4-interacting transcriptional co-activator; Transcription factor SMIF

Gene ID (Human)

55802

SwissProt (Human)

Q9NPI6

Available Sizes

Curated Selection

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