Repo-Man rabbit pAb

Cell division cycle associated 2 (CDCA2) Homo sapiens This gene encodes a targeting subunit of the cell-cycle associated protein, protein phosphatase 1, with a role in targeting this protein to chromatin during anaphase. These two proteins comprise a phosphatase complex that is involved in nuclear envelope reformation and regulation of the DNA damage response. The encoded protein may also play a role in cancer progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015],

Product Specifications

Background

UniProt

Q69YH5

Swiss Prot

Q69YH5

Reactivity

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CDCA2. AA range:511-560

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

120-130kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

120-130kD

Fragment

IgG

Subcellular Location

Nucleus . Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.