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PRIM1 rabbit pAb

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008],

Product Specifications

Background

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]

Synonyms

PRIM1; DNA primase small subunit; DNA primase 49 kDa subunit; p49

Gene ID

5557

UniProt

P49642

Cellular Locus

Nucleoplasm,alpha DNA polymerase:primase complex,membrane,primosome complex,

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human PRIM1. AA range:371-420

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight

50kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES3260-1.pdf

Observed Molecular Weight

50 kD

Subcellular Location

Nucleoplasm, alpha DNA polymerase:primase complex, membrane, primosome complex

Other Product Names

PRIM1; DNA primase small subunit; DNA primase 49 kDa subunit; p49

Gene ID (Human)

5557

SwissProt (Human)

P49642

Available Sizes

Curated Selection

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