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Periphilin 1 rabbit pAb

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Product Specifications

Background

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Synonyms

PPHLN1; HSPC206; HSPC232; Periphilin-1; Gastric cancer antigen Ga50

Gene ID

51535

UniProt

Q8NEY8

Cellular Locus

Nucleus. Cytoplasm. Chromosome. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416)..

Host

Rabbit

Species Reactivity

Human,Mouse

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human PPHLN. AA range:71-120

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

53kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES3175-1.pdf

Observed Molecular Weight

53 kD

Subcellular Location

Nucleus . Cytoplasm . Chromosome . In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457) . Localizes to chromatin (PubMed:26022416) . .

Other Product Names

PPHLN1; HSPC206; HSPC232; Periphilin-1; Gastric cancer antigen Ga50

Gene ID (Human)

51535

SwissProt (Human)

Q8NEY8

Available Sizes

Curated Selection

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