PCNA rabbit pAb

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P12004

Swiss Prot

P12004

Reactivity

Human; Mouse; Rat; Monkey; Rabbit

Immunogen

The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:100-500; IF/ICC 1:100-500; ELISA 1:5000-20000

Molecular Weight

30-33kd

Storage Conditions

-20°C/1 year

Observed Molecular Weight

30-33kd

Fragment

IgG

Subcellular Location

Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902) . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136) . Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136) . Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .