Myp rabbit pAb

NOL3 encodes an anti-apoptotic protein nucleolar protein 3 that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for NOL3.

Product Specifications

Background

Synonyms

Myp; NOL3; Nop30; Nucleolar protein 3; apoptosis repressor ARC; apoptosis repressor with CARD; apoptosis repressor with caspase recruitment domain (CARD); muscle-enriched cytoplasmic protein; nucleolar protein of 30 kDa

Gene ID

8996

UniProt

O60936-2

Cellular Locus

[Isoform 1]: Nucleus, nucleolus. The SR-rich C-terminus mediates nuclear localization..; [Isoform 3]: Cytoplasm.; [Isoform 2]: Cytoplasm. Mitochondrion. Sarcoplasmic reticulum. Membrane ; Lipid-anchor. Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress..

Host

Rabbit

Species Reactivity

Human,Rat,Mouse,

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human ARC. AA range:159-208

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

28kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES2881-1.pdf

Observed Molecular Weight

28 kD

Subcellular Location

[Isoform 1]: Nucleus, nucleolus . The SR-rich C-terminus mediates nuclear localization. .; [Isoform 3]: Cytoplasm .; [Isoform 2]: Cytoplasm . Mitochondrion . Sarcoplasmic reticulum . Membrane ; Lipid-anchor . Phosphorylation at Thr-149 results in translocation to mitochondria. Colocalized with mitochondria in response to oxidative stress. .

Other Product Names

Myp; NOL3; Nop30; Nucleolar protein 3; apoptosis repressor ARC; apoptosis repressor with CARD; apoptosis repressor with caspase recruitment domain (CARD) ; muscle-enriched cytoplasmic protein; nucleolar protein of 30 kDa

Gene ID (Human)

8996

SwissProt (Human)