MAD2 rabbit pAb

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

Q13257

Swiss Prot

Q13257

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human MAD2L1. AA range:91-140

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IP; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IP 1:200-500 ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

25kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

25kD

Fragment

IgG

Subcellular Location

Nucleus . Chromosome, centromere, kinetochore. Cytoplasm . Cytoplasm, cytoskeleton, spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable) . Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. Sequestered to the cytoplasm upon interaction with isoform 3 of MAD1L1 (PubMed:19010891) . .