Anti-RICK (CT) Antibody

Rabbit Polyclonal Antibody specific to RICK (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.11.1, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, Tyrosine-protein kinase RIPK2, EC 2.7.10.2

Gene Name

RIPK2

Gene ID

8767

NCBI Gene ID

UniProt

O43353

Accession Number

NP_003812

Cellular Locus

Cytoplasm

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 508-522 of human RICK (accession no. AF027706).

Target Antigen

Receptor-interacting serine/threonine-protein kinase 2

Target

RICK (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed:26646181). {PubMed:14638696, PubMed:17054981, PubMed:18079694, PubMed:21123652, PubMed:21887730, PubMed:26646181}.

Additionnal Information

Immunoblotting : use at 1-2ug/mL. <br><br>Positive control: A431 cell lysate. <br><br>Immunocytochemistry: use at 5ug/mL.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat RICK (60kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A CARD-containing serine/threonine kinase has been identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induces apoptosis and activates NF- B and JNK. RICK interacts with members of the TRAF family, CLARP, and caspase-1. RICK regulates TNF and Fas induced-apoptosis and is involved in the generation of the proinflammatory cytokine IL-1 . The mRNA of RICK is expressed in many human tissues.