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HXK I rabbit pAb

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],

Product Specifications

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]

Synonyms

HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Gene ID

3098

UniProt

P19367

Cellular Locus

Mitochondrion outer membrane ; Peripheral membrane protein. Cytoplasm, cytosol. The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331)..

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human HXK1. AA range:31-80

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

109kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES2587-1.pdf

Observed Molecular Weight

109 kD

Subcellular Location

Mitochondrion outer membrane ; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable) . Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331) . .

Other Product Names

HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Gene ID (Human)

3098

SwissProt (Human)

P19367

Available Sizes

Curated Selection

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