HXK I rabbit pAb

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],

Product Specifications

Background

UniProt

P19367

Swiss Prot

P19367

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human HXK1. AA range:31-80

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

109kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

109kD

Fragment

IgG

Subcellular Location

Mitochondrion outer membrane ; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable) . Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331) . .