Gα t1 rabbit pAb

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009],

Product Specifications

Background

Product Name Alternative

GNAT1; GNATR; Guanine nucleotide-binding protein G (t) subunit alpha-1; Transducin alpha-1 chain

UniProt

P11488

Swiss Prot

P11488

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human GNAT1. AA range:71-120

Target

Gα t1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

36kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

36kD

Fragment

IgG

Subcellular Location

Cell projection, cilium, photoreceptor outer segment . Membrane ; Peripheral membrane protein . Photoreceptor inner segment . Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light. .