FIR rabbit pAb

Function: Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. similarity: Contains 1 DH (DBL-homology) domain. similarity: Contains 1 FERM domain. similarity: Contains 2 PH domains. subunit: Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding.

Product Specifications

Background

Function:Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton., similarity:Contains 1 DH (DBL-homology) domain., similarity:Contains 1 FERM domain., similarity:Contains 2 PH domains., subunit:Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding.

UniProt

O94887

Swiss Prot

O94887

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human FIR. AA range:331-380

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

119kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

119kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytosol, cytoskeleton, extrinsic component of membrane

Other Product Names

FARP2; KIAA0793; PLEKHC3; FERM; RhoGEF and pleckstrin domain-containing protein 2; FERM domain including RhoGEF; FIR; Pleckstrin homology domain-containing family C member 3; PH domain-containing family C member 3