Dok-3 rabbit pAb

Domain: PTB domain mediates receptor interaction. function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function. PTM: Constitutively tyrosine-phosphorylated. PTM: On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase. similarity: Belongs to the DOK family. Type A subfamily. similarity: Contains 1 IRS-type PTB domain. similarity: Contains 1 PH domain. subunit: On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP. tissue specificity: Expressed in spleen.

Product Specifications

Background

Domain:PTB domain mediates receptor interaction., function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function., PTM:Constitutively tyrosine-phosphorylated., PTM:On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase., similarity:Belongs to the DOK family. Type A subfamily., similarity:Contains 1 IRS-type PTB domain., similarity:Contains 1 PH domain., subunit:On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP., tissue specificity:Expressed in spleen.

UniProt

Q7L591

Swiss Prot

Q7L591

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human DOK3. AA range:101-150

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.