DDX3 rabbit pAb

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarit

Product Specifications

Background

UniProt

O00571

Swiss Prot

O00571

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human DDX3/DEAD-box Protein 3. AA range:466-515

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight

73kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

73kD

Fragment

IgG

Subcellular Location

Cell membrane . Nucleus . Cytoplasm . Cytoplasm, Stress granule . Inflammasome . Cell projection, lamellipodium . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Shuttles between the nucleus and the cytosol (PubMed:15507209, PubMed:18636090, PubMed:29899501, PubMed:31575075, PubMed:30131165) . Exported from the nucleus partly through the XPO1/CRM1 system and partly through NXF1/TAP (PubMed:15507209, PubMed:18636090, PubMed:18596238, PubMed:31575075, PubMed:30131165) . Localizes to nuclear pores on the outer side of the nuclear membrane (PubMed:15507209) . In the cytosol, partly colocalizes with mitochondria (PubMed:20127681) . At G0, predominantly located in nucleus. In G1/S phase, predominantly cytoplasmic (PubMed:22034099) . During prophase/prometaphase, localizes in clos