BIG2 rabbit pAb

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

ARFGEF2; ARFGEP2; BIG2; Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Brefeldin A-inhibited GEP 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2

UniProt

Q9Y6D5

Swiss Prot

Q9Y6D5

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human ARFGEF2. AA range:1491-1540

Target

BIG2

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

210kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

210kD

Fragment

IgG

Subcellular Location

Cytoplasm. Membrane. Golgi apparatus. Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network . Endosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, dendrite . Cytoplasmic vesicle . Cell junction, synapse . Cytoplasm, cytoskeleton . Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.

Other Product Names

ARFGEF2; ARFGEP2; BIG2; Brefeldin A-inhibited guanine nucleotide-exchange protein 2; Brefeldin A-inhibited GEP 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2