AIRE-1 rabbit pAb

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012],

Product Specifications

Background

Product Name Alternative

AIRE; APECED; Autoimmune regulator; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; APECED protein

UniProt

O43918

Swiss Prot

O43918

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human AIRE. AA range:91-140

Target

AIRE-1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

60kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

60kD

Fragment

IgG

Subcellular Location

Nucleus . Cytoplasm . Predominantly nuclear but also cytoplasmic (PubMed:11274163, PubMed:14974083) . Found in nuclear body-like structures (dots) and in a filamentous vimentin-like pattern (PubMed:11274163, PubMed:14974083, PubMed:26084028) . Associated with tubular structures (PubMed:11274163, PubMed:14974083) . .