HSP90β (phospho Ser254) rabbit pAb

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],

Product Specifications

Background

UniProt

P08238

Swiss Prot

P08238

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight

90kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

90kD

Fragment

IgG

Subcellular Location

Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) . Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673) . Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762) . .