Stat1 (phospho Tyr701) rabbit pAb

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

P42224

Swiss Prot

P42224

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717

Clonality

Polyclonal

Source

Rabbit

Applications

IF; WB; IHC; IP; ELISA

Concentration

1 mg/ml

Dilution

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight

87kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

87kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4 (PubMed:15322115) . Monomethylation at Lys-525 is required for phosphorylation at Tyr-701 and translocation into the nucleus (PubMed:28753426) . Translocates into the nucleus in response to interferon-beta stimulation (PubMed:26479788) . .