Paxillin (phospho Tyr118) rabbit pAb

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID: 9054445). [provided by RefSeq, Aug 2011],

Product Specifications

Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) . Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445) . [provided by RefSeq, Aug 2011]

Product Name Alternative

PXN; Paxillin

UniProt

P49023

Swiss Prot

P49023

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr118. AA range:85-134

Target

Paxillin

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

64kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

64kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389) . .