GSK3β (phospho Ser9) rabbit pAb

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009],

Product Specifications

Background

Product Name Alternative

GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

UniProt

P49841

Swiss Prot

P49841

Reactivity

Human; Mouse; Rat; Drosophila

Immunogen

The antiserum was produced against synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9. AA range:1-50

Target

GSK3β

Clonality

Polyclonal

Source

Rabbit

Applications

IF; WB; IHC; IP; ELISA

Concentration

1 mg/ml

Dilution

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

48kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

48kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Cell membrane . The phosphorylated form shows localization to cytoplasm and cell membrane (PubMed:20937854) . The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854) . .